rt-pcr products Search Results


recombinant plasmids with sla rt-pcr products  (MCLAB Inc)
90
MCLAB Inc recombinant plasmids with sla rt-pcr products
Recombinant Plasmids With Sla Rt Pcr Products, supplied by MCLAB Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rt-pcr+products/pm31561059-56-9-19?v=MCLAB+Inc
Average 90 stars, based on 1 article reviews
recombinant plasmids with sla rt-pcr products - by Bioz Stars, 2026-07
90/100 stars
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clcn1 rt-pcr products  (Promega)
90
Promega clcn1 rt-pcr products
Expression of alternatively spliced transcripts in mbnl2 morphants. (A) <t>RT-PCR</t> analysis using primers tnnt2F and tnnt2R3 (arrows) yields two main bands of 318 bp and 282 bp in control embryos (‘C’), whereas the 282 bp product is not detectable in the morphants (MO). Boxes depict the exon content of each product after sequencing. Exons 4 (green) and 6 (yellow) are alternatively spliced. (B) The normal tnnt2 alternative splicing pattern is shown at different developmental stages. RT-PCR analysis of control embryos at the 18-somite stage (1) and 24 hpf (2) shows the expression of a 318 bp product. 51 hpf (3), 76 hpf (4) and adult (5) controls express both isoforms, 318 bp and 282 bp. (C) RT-PCR analysis of <t>clcn1</t> . Primers clcn1-134F and clcn1-87R (arrows) produce two bands, one of 588 bp (including exon 18a) and one of 555 bp (excluding exon 18a), which are expressed at a ratio of 1:1.2 in control embryos, whereas, in the mbnl2 morphants, the 555 bp product is the predominant form, shifting the ratio to 1:1.98. Differences in exon content are shown in the boxes. Exon 18a (red) is alternatively spliced and encodes amino acids FTKLSPAGGVK.
Clcn1 Rt Pcr Products, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rt-pcr+products/pmc03097459-239-1-9?v=Promega
Average 90 stars, based on 1 article reviews
clcn1 rt-pcr products - by Bioz Stars, 2026-07
90/100 stars
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isolated rt-pcr product  (Microsynth ag)
90
Microsynth ag isolated rt-pcr product
Expression of alternatively spliced transcripts in mbnl2 morphants. (A) <t>RT-PCR</t> analysis using primers tnnt2F and tnnt2R3 (arrows) yields two main bands of 318 bp and 282 bp in control embryos (‘C’), whereas the 282 bp product is not detectable in the morphants (MO). Boxes depict the exon content of each product after sequencing. Exons 4 (green) and 6 (yellow) are alternatively spliced. (B) The normal tnnt2 alternative splicing pattern is shown at different developmental stages. RT-PCR analysis of control embryos at the 18-somite stage (1) and 24 hpf (2) shows the expression of a 318 bp product. 51 hpf (3), 76 hpf (4) and adult (5) controls express both isoforms, 318 bp and 282 bp. (C) RT-PCR analysis of <t>clcn1</t> . Primers clcn1-134F and clcn1-87R (arrows) produce two bands, one of 588 bp (including exon 18a) and one of 555 bp (excluding exon 18a), which are expressed at a ratio of 1:1.2 in control embryos, whereas, in the mbnl2 morphants, the 555 bp product is the predominant form, shifting the ratio to 1:1.98. Differences in exon content are shown in the boxes. Exon 18a (red) is alternatively spliced and encodes amino acids FTKLSPAGGVK.
Isolated Rt Pcr Product, supplied by Microsynth ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rt-pcr+products/pmc05416887-99-5-10?v=Microsynth+ag
Average 90 stars, based on 1 article reviews
isolated rt-pcr product - by Bioz Stars, 2026-07
90/100 stars
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race pcr products  (GATC Biotech)
90
GATC Biotech race pcr products
Expression of alternatively spliced transcripts in mbnl2 morphants. (A) <t>RT-PCR</t> analysis using primers tnnt2F and tnnt2R3 (arrows) yields two main bands of 318 bp and 282 bp in control embryos (‘C’), whereas the 282 bp product is not detectable in the morphants (MO). Boxes depict the exon content of each product after sequencing. Exons 4 (green) and 6 (yellow) are alternatively spliced. (B) The normal tnnt2 alternative splicing pattern is shown at different developmental stages. RT-PCR analysis of control embryos at the 18-somite stage (1) and 24 hpf (2) shows the expression of a 318 bp product. 51 hpf (3), 76 hpf (4) and adult (5) controls express both isoforms, 318 bp and 282 bp. (C) RT-PCR analysis of <t>clcn1</t> . Primers clcn1-134F and clcn1-87R (arrows) produce two bands, one of 588 bp (including exon 18a) and one of 555 bp (excluding exon 18a), which are expressed at a ratio of 1:1.2 in control embryos, whereas, in the mbnl2 morphants, the 555 bp product is the predominant form, shifting the ratio to 1:1.98. Differences in exon content are shown in the boxes. Exon 18a (red) is alternatively spliced and encodes amino acids FTKLSPAGGVK.
Race Pcr Products, supplied by GATC Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rt-pcr+products/pmc05613102-133-0-8?v=GATC+Biotech
Average 90 stars, based on 1 article reviews
race pcr products - by Bioz Stars, 2026-07
90/100 stars
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hastv rt-pcr product  (MWG-Biotech ag)
90
MWG-Biotech ag hastv rt-pcr product
Expression of alternatively spliced transcripts in mbnl2 morphants. (A) <t>RT-PCR</t> analysis using primers tnnt2F and tnnt2R3 (arrows) yields two main bands of 318 bp and 282 bp in control embryos (‘C’), whereas the 282 bp product is not detectable in the morphants (MO). Boxes depict the exon content of each product after sequencing. Exons 4 (green) and 6 (yellow) are alternatively spliced. (B) The normal tnnt2 alternative splicing pattern is shown at different developmental stages. RT-PCR analysis of control embryos at the 18-somite stage (1) and 24 hpf (2) shows the expression of a 318 bp product. 51 hpf (3), 76 hpf (4) and adult (5) controls express both isoforms, 318 bp and 282 bp. (C) RT-PCR analysis of <t>clcn1</t> . Primers clcn1-134F and clcn1-87R (arrows) produce two bands, one of 588 bp (including exon 18a) and one of 555 bp (excluding exon 18a), which are expressed at a ratio of 1:1.2 in control embryos, whereas, in the mbnl2 morphants, the 555 bp product is the predominant form, shifting the ratio to 1:1.98. Differences in exon content are shown in the boxes. Exon 18a (red) is alternatively spliced and encodes amino acids FTKLSPAGGVK.
Hastv Rt Pcr Product, supplied by MWG-Biotech ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rt-pcr+products/pm15522011-26-1-7?v=MWG-Biotech+ag
Average 90 stars, based on 1 article reviews
hastv rt-pcr product - by Bioz Stars, 2026-07
90/100 stars
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rt-pcr amplicon product  (Promega)
90
Promega rt-pcr amplicon product
Expression of alternatively spliced transcripts in mbnl2 morphants. (A) <t>RT-PCR</t> analysis using primers tnnt2F and tnnt2R3 (arrows) yields two main bands of 318 bp and 282 bp in control embryos (‘C’), whereas the 282 bp product is not detectable in the morphants (MO). Boxes depict the exon content of each product after sequencing. Exons 4 (green) and 6 (yellow) are alternatively spliced. (B) The normal tnnt2 alternative splicing pattern is shown at different developmental stages. RT-PCR analysis of control embryos at the 18-somite stage (1) and 24 hpf (2) shows the expression of a 318 bp product. 51 hpf (3), 76 hpf (4) and adult (5) controls express both isoforms, 318 bp and 282 bp. (C) RT-PCR analysis of <t>clcn1</t> . Primers clcn1-134F and clcn1-87R (arrows) produce two bands, one of 588 bp (including exon 18a) and one of 555 bp (excluding exon 18a), which are expressed at a ratio of 1:1.2 in control embryos, whereas, in the mbnl2 morphants, the 555 bp product is the predominant form, shifting the ratio to 1:1.98. Differences in exon content are shown in the boxes. Exon 18a (red) is alternatively spliced and encodes amino acids FTKLSPAGGVK.
Rt Pcr Amplicon Product, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rt-pcr+products/us07572628-1081-48-56?v=Promega
Average 90 stars, based on 1 article reviews
rt-pcr amplicon product - by Bioz Stars, 2026-07
90/100 stars
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target rt-pcr products of the genes  (GenScript corporation)
90
GenScript corporation target rt-pcr products of the genes
Expression of alternatively spliced transcripts in mbnl2 morphants. (A) <t>RT-PCR</t> analysis using primers tnnt2F and tnnt2R3 (arrows) yields two main bands of 318 bp and 282 bp in control embryos (‘C’), whereas the 282 bp product is not detectable in the morphants (MO). Boxes depict the exon content of each product after sequencing. Exons 4 (green) and 6 (yellow) are alternatively spliced. (B) The normal tnnt2 alternative splicing pattern is shown at different developmental stages. RT-PCR analysis of control embryos at the 18-somite stage (1) and 24 hpf (2) shows the expression of a 318 bp product. 51 hpf (3), 76 hpf (4) and adult (5) controls express both isoforms, 318 bp and 282 bp. (C) RT-PCR analysis of <t>clcn1</t> . Primers clcn1-134F and clcn1-87R (arrows) produce two bands, one of 588 bp (including exon 18a) and one of 555 bp (excluding exon 18a), which are expressed at a ratio of 1:1.2 in control embryos, whereas, in the mbnl2 morphants, the 555 bp product is the predominant form, shifting the ratio to 1:1.98. Differences in exon content are shown in the boxes. Exon 18a (red) is alternatively spliced and encodes amino acids FTKLSPAGGVK.
Target Rt Pcr Products Of The Genes, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rt-pcr+products/pmc04334893-156-15-28?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
target rt-pcr products of the genes - by Bioz Stars, 2026-07
90/100 stars
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the amplified rt-pcr products, of 0.5 kb each (fbqr11fbqr14)  (Biosure Inc)
90
Biosure Inc the amplified rt-pcr products, of 0.5 kb each (fbqr11fbqr14)
Expression of alternatively spliced transcripts in mbnl2 morphants. (A) <t>RT-PCR</t> analysis using primers tnnt2F and tnnt2R3 (arrows) yields two main bands of 318 bp and 282 bp in control embryos (‘C’), whereas the 282 bp product is not detectable in the morphants (MO). Boxes depict the exon content of each product after sequencing. Exons 4 (green) and 6 (yellow) are alternatively spliced. (B) The normal tnnt2 alternative splicing pattern is shown at different developmental stages. RT-PCR analysis of control embryos at the 18-somite stage (1) and 24 hpf (2) shows the expression of a 318 bp product. 51 hpf (3), 76 hpf (4) and adult (5) controls express both isoforms, 318 bp and 282 bp. (C) RT-PCR analysis of <t>clcn1</t> . Primers clcn1-134F and clcn1-87R (arrows) produce two bands, one of 588 bp (including exon 18a) and one of 555 bp (excluding exon 18a), which are expressed at a ratio of 1:1.2 in control embryos, whereas, in the mbnl2 morphants, the 555 bp product is the predominant form, shifting the ratio to 1:1.98. Differences in exon content are shown in the boxes. Exon 18a (red) is alternatively spliced and encodes amino acids FTKLSPAGGVK.
The Amplified Rt Pcr Products, Of 0.5 Kb Each (Fbqr11fbqr14), supplied by Biosure Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rt-pcr+products/pm16741869-49-8-28?v=Biosure+Inc
Average 90 stars, based on 1 article reviews
the amplified rt-pcr products, of 0.5 kb each (fbqr11fbqr14) - by Bioz Stars, 2026-07
90/100 stars
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rt-pcr products  (AniCon Labor GmbH)
90
AniCon Labor GmbH rt-pcr products
Expression of alternatively spliced transcripts in mbnl2 morphants. (A) <t>RT-PCR</t> analysis using primers tnnt2F and tnnt2R3 (arrows) yields two main bands of 318 bp and 282 bp in control embryos (‘C’), whereas the 282 bp product is not detectable in the morphants (MO). Boxes depict the exon content of each product after sequencing. Exons 4 (green) and 6 (yellow) are alternatively spliced. (B) The normal tnnt2 alternative splicing pattern is shown at different developmental stages. RT-PCR analysis of control embryos at the 18-somite stage (1) and 24 hpf (2) shows the expression of a 318 bp product. 51 hpf (3), 76 hpf (4) and adult (5) controls express both isoforms, 318 bp and 282 bp. (C) RT-PCR analysis of <t>clcn1</t> . Primers clcn1-134F and clcn1-87R (arrows) produce two bands, one of 588 bp (including exon 18a) and one of 555 bp (excluding exon 18a), which are expressed at a ratio of 1:1.2 in control embryos, whereas, in the mbnl2 morphants, the 555 bp product is the predominant form, shifting the ratio to 1:1.98. Differences in exon content are shown in the boxes. Exon 18a (red) is alternatively spliced and encodes amino acids FTKLSPAGGVK.
Rt Pcr Products, supplied by AniCon Labor GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rt-pcr+products/pmc07429401-91-2-7?v=AniCon+Labor+GmbH
Average 90 stars, based on 1 article reviews
rt-pcr products - by Bioz Stars, 2026-07
90/100 stars
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epha6 epha10 rt-pcr products  (Promega)
90
Promega epha6 epha10 rt-pcr products
Expression of alternatively spliced transcripts in mbnl2 morphants. (A) <t>RT-PCR</t> analysis using primers tnnt2F and tnnt2R3 (arrows) yields two main bands of 318 bp and 282 bp in control embryos (‘C’), whereas the 282 bp product is not detectable in the morphants (MO). Boxes depict the exon content of each product after sequencing. Exons 4 (green) and 6 (yellow) are alternatively spliced. (B) The normal tnnt2 alternative splicing pattern is shown at different developmental stages. RT-PCR analysis of control embryos at the 18-somite stage (1) and 24 hpf (2) shows the expression of a 318 bp product. 51 hpf (3), 76 hpf (4) and adult (5) controls express both isoforms, 318 bp and 282 bp. (C) RT-PCR analysis of <t>clcn1</t> . Primers clcn1-134F and clcn1-87R (arrows) produce two bands, one of 588 bp (including exon 18a) and one of 555 bp (excluding exon 18a), which are expressed at a ratio of 1:1.2 in control embryos, whereas, in the mbnl2 morphants, the 555 bp product is the predominant form, shifting the ratio to 1:1.98. Differences in exon content are shown in the boxes. Exon 18a (red) is alternatively spliced and encodes amino acids FTKLSPAGGVK.
Epha6 Epha10 Rt Pcr Products, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rt-pcr+products/pmc04404412-152-1-10?v=Promega
Average 90 stars, based on 1 article reviews
epha6 epha10 rt-pcr products - by Bioz Stars, 2026-07
90/100 stars
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rt-pcr product  (NORTHWOODS DNA)
90
NORTHWOODS DNA rt-pcr product
Expression of alternatively spliced transcripts in mbnl2 morphants. (A) <t>RT-PCR</t> analysis using primers tnnt2F and tnnt2R3 (arrows) yields two main bands of 318 bp and 282 bp in control embryos (‘C’), whereas the 282 bp product is not detectable in the morphants (MO). Boxes depict the exon content of each product after sequencing. Exons 4 (green) and 6 (yellow) are alternatively spliced. (B) The normal tnnt2 alternative splicing pattern is shown at different developmental stages. RT-PCR analysis of control embryos at the 18-somite stage (1) and 24 hpf (2) shows the expression of a 318 bp product. 51 hpf (3), 76 hpf (4) and adult (5) controls express both isoforms, 318 bp and 282 bp. (C) RT-PCR analysis of <t>clcn1</t> . Primers clcn1-134F and clcn1-87R (arrows) produce two bands, one of 588 bp (including exon 18a) and one of 555 bp (excluding exon 18a), which are expressed at a ratio of 1:1.2 in control embryos, whereas, in the mbnl2 morphants, the 555 bp product is the predominant form, shifting the ratio to 1:1.98. Differences in exon content are shown in the boxes. Exon 18a (red) is alternatively spliced and encodes amino acids FTKLSPAGGVK.
Rt Pcr Product, supplied by NORTHWOODS DNA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rt-pcr+products/pmc03261624-58-1-12?v=NORTHWOODS+DNA
Average 90 stars, based on 1 article reviews
rt-pcr product - by Bioz Stars, 2026-07
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rt-pcr products  (DWK Life Sciences)
90
DWK Life Sciences rt-pcr products
Expression of alternatively spliced transcripts in mbnl2 morphants. (A) <t>RT-PCR</t> analysis using primers tnnt2F and tnnt2R3 (arrows) yields two main bands of 318 bp and 282 bp in control embryos (‘C’), whereas the 282 bp product is not detectable in the morphants (MO). Boxes depict the exon content of each product after sequencing. Exons 4 (green) and 6 (yellow) are alternatively spliced. (B) The normal tnnt2 alternative splicing pattern is shown at different developmental stages. RT-PCR analysis of control embryos at the 18-somite stage (1) and 24 hpf (2) shows the expression of a 318 bp product. 51 hpf (3), 76 hpf (4) and adult (5) controls express both isoforms, 318 bp and 282 bp. (C) RT-PCR analysis of <t>clcn1</t> . Primers clcn1-134F and clcn1-87R (arrows) produce two bands, one of 588 bp (including exon 18a) and one of 555 bp (excluding exon 18a), which are expressed at a ratio of 1:1.2 in control embryos, whereas, in the mbnl2 morphants, the 555 bp product is the predominant form, shifting the ratio to 1:1.98. Differences in exon content are shown in the boxes. Exon 18a (red) is alternatively spliced and encodes amino acids FTKLSPAGGVK.
Rt Pcr Products, supplied by DWK Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rt-pcr+products/10__1080_slash_17429145__2011__591945-54-5-49?v=DWK+Life+Sciences
Average 90 stars, based on 1 article reviews
rt-pcr products - by Bioz Stars, 2026-07
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Image Search Results


Expression of alternatively spliced transcripts in mbnl2 morphants. (A) RT-PCR analysis using primers tnnt2F and tnnt2R3 (arrows) yields two main bands of 318 bp and 282 bp in control embryos (‘C’), whereas the 282 bp product is not detectable in the morphants (MO). Boxes depict the exon content of each product after sequencing. Exons 4 (green) and 6 (yellow) are alternatively spliced. (B) The normal tnnt2 alternative splicing pattern is shown at different developmental stages. RT-PCR analysis of control embryos at the 18-somite stage (1) and 24 hpf (2) shows the expression of a 318 bp product. 51 hpf (3), 76 hpf (4) and adult (5) controls express both isoforms, 318 bp and 282 bp. (C) RT-PCR analysis of clcn1 . Primers clcn1-134F and clcn1-87R (arrows) produce two bands, one of 588 bp (including exon 18a) and one of 555 bp (excluding exon 18a), which are expressed at a ratio of 1:1.2 in control embryos, whereas, in the mbnl2 morphants, the 555 bp product is the predominant form, shifting the ratio to 1:1.98. Differences in exon content are shown in the boxes. Exon 18a (red) is alternatively spliced and encodes amino acids FTKLSPAGGVK.

Journal: Disease Models & Mechanisms

Article Title: Zebrafish deficient for Muscleblind-like 2 exhibit features of myotonic dystrophy

doi: 10.1242/dmm.004150

Figure Lengend Snippet: Expression of alternatively spliced transcripts in mbnl2 morphants. (A) RT-PCR analysis using primers tnnt2F and tnnt2R3 (arrows) yields two main bands of 318 bp and 282 bp in control embryos (‘C’), whereas the 282 bp product is not detectable in the morphants (MO). Boxes depict the exon content of each product after sequencing. Exons 4 (green) and 6 (yellow) are alternatively spliced. (B) The normal tnnt2 alternative splicing pattern is shown at different developmental stages. RT-PCR analysis of control embryos at the 18-somite stage (1) and 24 hpf (2) shows the expression of a 318 bp product. 51 hpf (3), 76 hpf (4) and adult (5) controls express both isoforms, 318 bp and 282 bp. (C) RT-PCR analysis of clcn1 . Primers clcn1-134F and clcn1-87R (arrows) produce two bands, one of 588 bp (including exon 18a) and one of 555 bp (excluding exon 18a), which are expressed at a ratio of 1:1.2 in control embryos, whereas, in the mbnl2 morphants, the 555 bp product is the predominant form, shifting the ratio to 1:1.98. Differences in exon content are shown in the boxes. Exon 18a (red) is alternatively spliced and encodes amino acids FTKLSPAGGVK.

Article Snippet: Additionally, clcn1 RT-PCR products were cloned into T-easy vector (Promega).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Sequencing

The mbnl2 morphant phenotype can be rescued by mbnl2 and MBNL2 mRNAs, in a dose-dependent manner. (A) The phenotype induced by MO-1 is rescued by co-injection with mbnl2 mRNA. The histogram shows the frequency of the observed phenotype after injection of 4.28 ng MO-1 or the same amount of MO-2 ( n =100, from five independent experiments). The percentage of affected embryos is significantly reduced when 150 pg mbnl2 mRNA is co-injected with 4.28 ng MO-1. (B,C) The completely rescued embryos (B) are indistinguishable from the controls (C). (D) The partially rescued embryos show a mild phenotype consisting of the presence of slight eye pigmentation defects, minor heart hypoplasia, mild spinal defects, and a head that is protuberant at the fourth ventricle level of the hindbrain. (E,F) The normal splicing pattern of tnnt2 (E) and clcn1 (F) is restored by co-injection of MO-1 with mbnl2 mRNA. The ratio of the upper and lower band is 1:2 in the morphants (MO), whereas, in controls (CT), it is 1:1.46, and is 1:1.34 in the rescued embryos (R). When co-injecting the human homologue, 75 pg of MBNL2 mRNA is the most effective dose in compensating the phenotype induced by MO-1 .

Journal: Disease Models & Mechanisms

Article Title: Zebrafish deficient for Muscleblind-like 2 exhibit features of myotonic dystrophy

doi: 10.1242/dmm.004150

Figure Lengend Snippet: The mbnl2 morphant phenotype can be rescued by mbnl2 and MBNL2 mRNAs, in a dose-dependent manner. (A) The phenotype induced by MO-1 is rescued by co-injection with mbnl2 mRNA. The histogram shows the frequency of the observed phenotype after injection of 4.28 ng MO-1 or the same amount of MO-2 ( n =100, from five independent experiments). The percentage of affected embryos is significantly reduced when 150 pg mbnl2 mRNA is co-injected with 4.28 ng MO-1. (B,C) The completely rescued embryos (B) are indistinguishable from the controls (C). (D) The partially rescued embryos show a mild phenotype consisting of the presence of slight eye pigmentation defects, minor heart hypoplasia, mild spinal defects, and a head that is protuberant at the fourth ventricle level of the hindbrain. (E,F) The normal splicing pattern of tnnt2 (E) and clcn1 (F) is restored by co-injection of MO-1 with mbnl2 mRNA. The ratio of the upper and lower band is 1:2 in the morphants (MO), whereas, in controls (CT), it is 1:1.46, and is 1:1.34 in the rescued embryos (R). When co-injecting the human homologue, 75 pg of MBNL2 mRNA is the most effective dose in compensating the phenotype induced by MO-1 .

Article Snippet: Additionally, clcn1 RT-PCR products were cloned into T-easy vector (Promega).

Techniques: Injection